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SOX9 Mouse Monoclonal Antibody

Cat Number: MABN81073
Conjugate: Unconjugated
Size: 100ul
Concentration: 1mg/ml
Host: Mouse
Isotype: Mouse IgG1
Immunogen: Purified recombinant fragment of human SOX9 expressed in E. Coli.
Reactivity: Human
Applications: WB 1:500-1:2000,ICC 1:200-1:1000,ELISA 1:5000-1:20000
Molecular: 56kDa
Purification: Affinity purification
Synonyms: CMD1; SRA1; CMPD1
Background:

The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal.

Form: liquid
Buffer: Purified antibody in PBS with 0.05% sodium azide
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Western blot analysis using SOX9 mouse mAb against Lovo (1) and SW620 (2) cell lysate.

Western blot analysis using SOX9 mouse mAb against Lovo (1) and SW620 (2) cell lysate.

Immunofluorescence analysis of Hela cells using SOX9 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunofluorescence analysis of Hela cells using SOX9 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.