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SOX9

Cat Number: AB-80691
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: A synthetic peptide of human SOX9
Reactivity: Hu,Ms,Rt
Applications:

Western Blot: 1:1000

Molecular: 60-66kDa
Purification: Aff. Pur.
Background:

The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal.

Form: Liquid
Buffer: Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
AB-80691-1.jpg

Western blot analysis of extracts of
various cell lines, using SOX9
antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST.
Detection: ECL West Pico Plus.
Exposure time: 40s.

AB-80691-2.jpg

Western blot analysis of extracts of
HepG2 cells, using SOX9 antibody
at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST.
Detection: ECL West Pico Plus.
Exposure time: 120s.