Sp1 (I18)
| Cat Number: | AB-10269 |
|---|---|
| Size: | 200 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | A synthesized peptide derived from human SP1. |
| Reactivity: | Ms, Rt, Hu |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular Weight: | 90kDa |
| Purification: | Aff. Pur. |
| Background: | The protein encoded by this gene is a zinc finger transcription factor that binds to GCrich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA,50% glycerol, pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of various cell lines, using SP1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 90s.
Immunohistochemistry of paraffin embedded
rat spleen using SP1 antibody
at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin
Embedded mouse kidney using SP1
antibody at dilution of 1:100
(40x lens).
Immunofluorescence analysis of C6 cells
using SP1 antibody.
Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3
cells using SP1 antibody.
Blue: DAPI for nuclear staining.