Splicing Factor 1 Rabbit Polyclonal Antibody

Cat Number: AB-84791
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rabbit
Isotype: IgG
Immunogen: The antiserum was produced against synthesized peptide derived from human SF1. AA range:48-97
Reactivity: Human;Mouse

Western Blot: 1/500 – 1/2000.
Immunohistochemistry: 1/100 – 1/300
Immunofluorescence: 1/200 – 1/1000

Molecular Weight: 68kD
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms: SF1; ZFM1; ZNF162; Splicing factor 1; Mammalian branch point-binding protein; BBP; mBBP; Transcription factor ZFM1; Zinc finger gene in MEN1 locus; Zinc finger protein 162

This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3′ splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants.

Form: Liquid
Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western Blot analysis of various cells using Splicing factor 1 Polyclonal Antibody diluted at 1:500


Western blot analysis of lysates from COLO205 cells, using SF1 Antibody. The lane on the right is blocked with the
synthesized peptide.


Immunohistochemistry analysis of paraffin-embedded human brain tissue, using SF1 Antibody. The picture on the right is blocked with the synthesized peptide.


Immunofluorescence analysis of HeLa cells, using SF1 Antibody. The picture on the right is blocked with the synthesized peptide.