SQSTM1 / P62
| Cat Number: | AB-J14285 |
|---|---|
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Synthesized peptide derived from human SQSTM |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500-2000 |
| Molecular Weight: | 62kDa |
| Purification: | Aff. Pur. |
| Background: | SQSTM1(Sequestosome-1), also known as Ubiquitin-Binding Protein P62 or P62, is a protein that in humans is encoded by the SQSTM1 gene. The Src homology type 2(SH2) domain is a highly conserved motif of about 100 amino acids which mediates protein-protein interactions by binding to phosphotyrosine.p56-lck, a T-cell-specific src family tyrosine kinase with an SH2 domain, is involved in T-cell signal transduction. The International Radiation Hybrid Mapping Consortium mapped the p62 gene to chromosome 5q35. Park et al.(1995) found that the p56-lck SH2 domain binds to p62 at the ser59 of p62 only when that serine is phosphorylated. Joung et al.(1996) expressed epitope-tagged p62 in Hela cells and showed that the expressed protein bound to the lck SH2 domain and that this binding was dependent on the N-terminal 50 amino acids of p62 but not on the tyrosine residue in this region. |
| Form: | Liquid |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage: | At -20˚C for one year. at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
Anti-SQSTM1/p62 antibody, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: HELA Cell Lysate
Lane 3: U87 Cell Lysate
Lane 4: A549 Cell Lysate
Immunofluorescence analysis of Hela cells treated or un-treated with UV 30MIN. 1,P62 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2,
AFlour 594 labled Secondary antibody was diluted at 1:300(room temperature, 50min).
.