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SQSTM1/ P62

Cat Number: AB-84085/AB-83779
Conjugate: Unconjugated
Size: 100ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-440 of human P62/SQSTM1
Reactivity: Hu, Ms, Rt
Applications:

Western Blot: 1:500-1:2000
Immunohistochemistry (paraffin-embedded tissues): 1:50 – 1:200
Immunofluorescence: 1:50 – 1:200
Immunoprecipitation: 1:50 – 1:200

Molecular: 62kDa
Purification: Aff. Pur.
Background:

This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.

Form: Liquid
Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles
AB-83779-1.jpg

Western blot analysis of extracts of various cell lines, using 62/SQSTM1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Kit.
Exposure time: 10s.

AB-83779-2.jpg

Western blot analysis of extracts of various cell lines, using SQSTM1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic KitExposure time: 30s.

AB-83779-3.jpg

Immunohistochemistry of paraffin embedded
human colon carcinoma using
SQSTM1 / p62 antibody at
dilution of 1:100 (40x lens).

AB-83779-4.jpg

Immunohistochemistry of paraffin embedded human tonsil using SQSTM1 / p62 antibody at dilution of 1:150
(40x lens).

AB-83779-5.jpg

Immunofluorescence analysis of HeLa cells
using SQSTM1 / p62 antibody at
dilution of 1:100. Blue: DAPI for nuclear
staining.

AB-83779-6.jpg

Immunofluorescence analysis of NIH/3T3 cells using SQSTM1 / p62 antibody at dilution of 1:100. Blue: DAPI
for nuclear staining.

References

References for SQSTM1 / p62 Rabbit pAb
Product:SQSTM1 / p62 Rabbit pAb
Journal:Redox Biology
Application:WB
IF:7.79
Species:Homo sapiens
PMID:31785531
Title:HEATR1 deficiency promotes pancreatic cancer proliferation and gemcitabine resistance by up-regulating Nrf2 signaling

References for SQSTM1 / p62 Rabbit pAb
Product:SQSTM1 / p62 Rabbit pAb
Journal:Insect Biochemistry and Molecular Biology
Application:WB
IF:3.61
Species:Bombyx mori
PMID:31654713
Title:Steroid hormone 20-hydroxyecdysone induces the transcription and complex assembly of V-ATPases to facilitate autophagy in Bombyx mori

References for SQSTM1 / p62 Rabbit pAb
Product: SQSTM1 / p62 Rabbit pAb
Journal:J Mol Med (Berl)
Application:WB
IF:4.42
Species:Mus musculus
PMID:32783081
Title:NLRP6 contributes to inflammation and brain injury following intracerebral haemorrhage by activating autophagy