SUMO3 Rabbit Polyclonal antibody
| Cat Number: | AB-84758 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-92 of human SUMO3. |
| Reactivity: | Human, Mouse, Rat |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular Weight: | 17-50kDa |
| Purification: | Affinity purification |
| Synonyms: | SMT3A; Smt3B; SMT3H1; SUMO-3 |
| Background: | This gene encodes a member of the small ubiquitin-related modifier (SUMO) family of eukaryotic proteins. The encoded protein is covalently conjugated to other proteins via a post-translation modification known as sumoylation. Sumoylation may play a role in a wide variety of cellular processes, including nuclear transport, DNA replication and repair, mitosis, transcriptional regulation, and signal transduction. Alternatively spliced transcript variants encoding distinct proteins have been described. |
| Form: | Liquid |
| Buffer: | PBS with 0.01% thiomersal,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of various cell lines, using SUMO3 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 90s.
Immunofluorescence analysis of U-2 OS cells using SUMO3 antibody at dilution of 1:100.
Blue: DAPI for nuclear staining.