TGF beta2 R
| Cat Number: | AB-81574 |
|---|---|
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 23-280 of human TGFBR2. |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500 -1:2000 |
| Molecular Weight: | 75kDa |
| Purification: | Aff. Pur. |
| Synonyms: | TGFBR2; AAT3; FAA3; LDS1B; LDS2; LDS2B; MFS2; RIIC; TAAD2; TGFR-2; TGFbeta-RII; TGF-beta rec eptor type-2. |
| Background: | This gene encodes a member of the Ser/Thr protein kinase family and the TGFB receptor subfamily. The encoded protein is a transmembrane protein that has a protein kinase domain, forms a heterodimeric complex with another receptor protein, and binds TGF-beta. This receptor/ligand complex phosphorylates proteins, which then enter the nucleus and regulate the transcription of a subset of genes related to cell proliferation. Mutations in this gene have been associated with Marfan Syndrome, Loeys-Deitz Aortic Aneurysm Syndrome, and the development of various types of tumors. Alternatively spliced transcript variants encoding different isoforms have been characterized. |
| Form: | Liquid |
Western blot analysis of extracts of
various cell lines, using TGFBR2
antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST.
Detection: ECL West Pico Plus.
Exposure time: 3s.
Immunohistochemistry of paraffin embedded
human appendix using
TGFBR2 antibody at dilution
of 1:100 (40x lens).
Immunohistochemistry of paraffin embedded
human breast cancer using
TGFBR2 antibody at dilution
of 1:100 (40x lens).
Immunofluorescence analysis of
NIH/3T3 cells using TGFBR2 antibody
at dilution of 1:100.
Blue: DAPI for nuclear staining.