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TGF beta2 R

Cat Number: AB-81574
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 23-280 of human TGFBR2.
Reactivity: Hu, Ms, Rt
Applications:

Western Blot: 1:500 -1:2000
Immunohistochemistry: 1:50 – 1:200
Immunofluorescence: 1:50 – 1:200

Molecular: 75kDa
Purification: Aff. Pur.
Synonyms: TGFBR2; AAT3; FAA3; LDS1B; LDS2; LDS2B; MFS2; RIIC; TAAD2; TGFR-2; TGFbeta-RII; TGF-beta rec eptor type-2.
Background:

This gene encodes a member of the Ser/Thr protein kinase family and the TGFB receptor subfamily. The encoded protein is a transmembrane protein that has a protein kinase domain, forms a heterodimeric complex with another receptor protein, and binds TGF-beta. This receptor/ligand complex phosphorylates proteins, which then enter the nucleus and regulate the transcription of a subset of genes related to cell proliferation. Mutations in this gene have been associated with Marfan Syndrome, Loeys-Deitz Aortic Aneurysm Syndrome, and the development of various types of tumors. Alternatively spliced transcript variants encoding different isoforms have been characterized.

Form: Liquid
AB-81574-1.jpg

Western blot analysis of extracts of
various cell lines, using TGFBR2
antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST.
Detection: ECL West Pico Plus.
Exposure time: 3s.

AB-81574-2.jpg

Immunohistochemistry of paraffin embedded
human appendix using
TGFBR2 antibody at dilution
of 1:100 (40x lens).

AB-81574-3.jpg

Immunohistochemistry of paraffin embedded
human breast cancer using
TGFBR2 antibody at dilution
of 1:100 (40x lens).

AB-81574-4.jpg

Immunofluorescence analysis of
NIH/3T3 cells using TGFBR2 antibody
at dilution of 1:100.
Blue: DAPI for nuclear staining.