TGN38
| Cat Number: | MAB-94619 |
|---|---|
| Size: | 100 ug |
| Clone: | TG21 |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | A synthesized peptide derived from human TGN46 |
| Reactivity: | Hu |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular Weight: | 90-100kDa |
| Purification: | Aff. Pur. |
| Synonyms: | TGN38; TGN46; TGN48; TGN51; TTGN2 |
| Background: | This gene encodes a type I integral membrane protein that is localized to the trans-Golgi network, a major sorting station for secretory and membrane proteins. The encoded protein cycles between early endosomes and the trans-Golgi network, and may play a role in exocytic vesicle formation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store for short term at 4-8°C .At -20°C for longer term. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of HeLa cells, using TGN38 Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus
Exposure time: 1s.
Immunohistochemistry of paraffin
Embedded human liver using TGN38
Rabbit mAb at dilution of 1:100
(40x lens).
Immunofluorescence analysis of U-2 OS
cells using TGN38 Rabbit mAb at
dilution of 1:100 (40x lens).
Blue: DAPI for nuclear staining.