Cat Number: MAB-94619
Size: 100 ug
Clone: TG21
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: A synthesized peptide derived from human TGN46
Reactivity: Hu

Western Blot: 1:500 – 1:2000
Immunohistochemistry: 1:50 – 1:200
Immunofluorescence: 1:50 – 1:200

Molecular Weight: 90-100kDa
Purification: Aff. Pur.
Synonyms: TGN38; TGN46; TGN48; TGN51; TTGN2

This gene encodes a type I integral membrane protein that is localized to the trans-Golgi network, a major sorting station for secretory and membrane proteins. The encoded protein cycles between early endosomes and the trans-Golgi network, and may play a role in exocytic vesicle formation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Form: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store for short term at 4-8°C .At -20°C for longer term. Avoid freeze / thaw cycles.

Western blot analysis of extracts of HeLa cells, using TGN38 Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus
Exposure time: 1s.


Immunohistochemistry of paraffin
Embedded human liver using TGN38
Rabbit mAb at dilution of 1:100
(40x lens).


Immunofluorescence analysis of U-2 OS
cells using TGN38 Rabbit mAb at
dilution of 1:100 (40x lens).
Blue: DAPI for nuclear staining.