Thrombospondin-1/THBS1
| Cat Number: | AB-83786 |
|---|---|
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 80-360 of human THBS1 |
| Reactivity: | Hu, Ms, |
| Applications: | Western Blot: 1:1000 |
| Molecular Weight: | 150kDa |
| Purification: | Aff. Pur. |
| Synonyms: | THBS;THBS-1;TSP;TSP-1;TSP1 |
| Background: | The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cellto- matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store for short term at 4-8°C .At -20°C for longer term. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of
various cell lines, using THBS1
antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.