TMEM119 Rabbit Polyclonal Antibody
| Cat Number: | AB-84816 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | Recombinant protein of human TMEM119. |
| Reactivity: | Mouse, Human |
| Applications: | Western Blot: 1:6000 – 1:24000 Immunocytochemistry: 1:500 – 1:2000 |
| Molecular Weight: | 56kDa |
| Purification: | Affinity Purified |
| Synonyms: | OBIF |
| Background: | Involved in positive regulation of bone mineralization; positive regulation of osteoblast differentiation; and positive regulation of osteoblast proliferation. Located in plasma membrane. |
| Form: | Liquid |
| Buffer: | PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of lysates from Mouse brain using TMEM119 Rabbit at 1:12000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 45s.
Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using TMEM119 Rabbit at a dilution of 1:6000 (40xlens). High pressure antigen retrieval
performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse intestine tissue using TMEM119 Rabbit at a dilution of 1:6000 (40xlens). High pressure antigen retrieval
performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Confocal imaging of paraffinembedded Mouse brain tissue usingTMEM119 Rabbitdilution 1:500) followed by a further incubation with Cy3 Goat Anti-Rabbit
IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M
Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.