TNF alpha Rabbit Polyclonal Antibody
| Cat Number: | AB-90222 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human TNFA. AA range:141-190. |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1/500 – 1/2000 |
| Molecular Weight: | 16kD |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Synonyms: | TNF; TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a |
| Background: | This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. |
| Form: | Liquid |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage: | Store at -20°C. Avoid repeated freeze-thaw cycles. |
Western blot analysis of THP-1, using TNF-α Rabbit pAb at 1:1000 dilution.THP-1 cells were
treated by PMA/TPA (80 nM) at 37℃ for overnight and LPS (1 μg/ml) at 37℃ for 6 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 60s.
Western Blot analysis of various cells using TNF-α Polyclonal Antibody.
Secondary antibody was diluted at 1:20000
Immunohistochemistry of paraffinembedded human colon carcinoma using TNF-α Rabbit pAb at dilution of
1:1000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffinembedded human liver using TNF-α Rabbit pAb at dilution of 1:1000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffinembedded human spleen using TNF-α Rabbit pAb at dilution of 1:1000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of A549.
1,primaryAntibody(red) was diluted at 1:200(4°C overnight).
2, GoatAnti Rabbit IgG (H&L) – Alexa Fluor 594 Secondaryantibody was diluted at 1:1000(room temperature,
50min).
3, Picture B: DAPI(blue) 10min.