|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 1-233 of human TNF.|
|Reactivity:||Hu, Ms, Rt|
Western Blot: 1:1000 – 1:2000
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases,including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
|Buffer:||PBS with 0.02% sodium azide,50% glycerol, pH7.3.|
Western blot analysis of extracts of various cell lines, using TNF-α antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL West Pico Plus.
Exposure time: 30s.
Immunohistochemistry of paraffin embedded Human colon carcinoma using TNF-α antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin embedded Human breast cancer using TNF-α antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin
Embedded Human gastric cancer
using TNF-α antibody at dilution
of 1:100 (40x lens).
Immunofluorescence analysis of Human mammary cancer cells using TNF-α antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Review for TNF-α Rabbit mAb
Experiment Type Western blot (WB)