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TNF alpha

Cat Number: MAB-94636
Size: 100 ug
Clone: TNF-3
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-233 of human TNF.
Reactivity: Hu, Ms, Rt
Applications:

Western Blot: 1:1000 – 1:2000
Immunohistochemistry: 1:50 – 1:200
Immunofluorescence: 1:50 – 1:200

Molecular: 17kDa
Purification: Aff. Pur.
Synonyms: TNF;DIF;TNF-alpha;TNFA;TNFSF2;TNLG1F;TNF alpha
Background:

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases,including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.

Form: Liquid
Buffer: PBS with 0.02% sodium azide,50% glycerol, pH7.3.
MAB-94636-1.jpg

Western blot analysis of extracts of various cell lines, using TNF-α antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL West Pico Plus.
Exposure time: 30s.

MAB-94636-2.jpg

Immunohistochemistry of paraffin embedded Human colon carcinoma using TNF-α antibody at dilution of 1:100 (40x lens).

MAB-94636-3.jpg

Immunohistochemistry of paraffin embedded Human breast cancer using TNF-α antibody at dilution of 1:100 (40x lens).

MAB-94636-4.jpg

Immunohistochemistry of paraffin
Embedded Human gastric cancer
using TNF-α antibody at dilution
of 1:100 (40x lens).

MAB-94636-5.jpg

Immunofluorescence analysis of Human mammary cancer cells using TNF-α antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

MAB-94636-6.jpg

Review:
Review for TNF-α Rabbit mAb
Experiment Type Western blot (WB)

References