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TNF alpha

Cat Number: AB-90222
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Recombinant protein of human TNF
Reactivity: Hu, Ms, Rt
Applications:

Western Blot 1:1000 – 1:2000
Immunohistochemistry (paraffin-embedded tissues): 1:50 – 1:200
Immunofluorescence:

Molecular: 27kDa
Purification: Aff. Pur.
Synonyms: TNF ; TNFSF2; DIF; Tumor necrosis factor; TNF a; TNF alpha
Background:

TNF-α, the prototypical member of the TNF protein superfamily, is a homotrimeric type-II membrane protein (1,2). Membrane-bound TNF-α is cleaved by the metalloprotease TACE/ADAM17 to generate a soluble homotrimer (2). Both membrane and soluble forms of TNF-α are biologically active. TNF-α is produced by a variety of immune cells including T cells, B cells, NK cells, and macrophages (1). Cellular response to TNF-α is mediated through interaction with receptors TNF-R1 and TNF-R2 and results in activation of pathways that favor both cell survival and apoptosis depending on the cell type and biological context. Activation of kinase pathways (including JNK, Erk (p44/42), p38 MAPK, and NF-κB) promotes the survival of cells, while TNF-α-mediated activation of caspase-8 leads to programmed cell death (1,2). TNF-α plays a key regulatory role in inflammation and host defense against bacterial infection, notably Mycobacterium tuberculosis (3). The role of TNF-α in autoimmunity is underscored by blocking TNF-α action to treat rheumatoid arthritis and Crohn’s disease (1,2,4).

Form: Liquid
Buffer: PBS with 0.02% sodium azide,50% glycerol, pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
AB-90222-1.jpg

Western blot analysis of extracts of various cell lines, using TNF antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

AB-90222-2.jpg

Immunohistochemistry of paraffin-embedded human gastric using TNF antibody at dilution of 1:100

AB-90222-3.jpg

Immunofluorescence analysis of RAW264.7 cells using TNF-α antibody at dilution of 1:100. Blue: DAPI for nuclear staining