Top

TNF alpha Rabbit Polyclonal Antibody

Cat Number: AB-90222
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: The antiserum was produced against synthesized peptide derived from human TNFA. AA range:141-190.
Reactivity: Hu, Ms, Rt
Applications:

Western Blot: 1/500 – 1/2000
Immuohistochemistry(paraffin-embedded tissues) 1:100-300
Immunofluorescence: 1:100-300
ELISA: 1/20000

Molecular Weight: 16kD
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms: TNF; TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
Background:

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.

Form: Liquid
Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage: Store at -20°C. Avoid repeated freeze-thaw cycles.
AB-90222-1

Western blot analysis of THP-1, using TNF-α Rabbit pAb at 1:1000 dilution.THP-1 cells were
treated by PMA/TPA (80 nM) at 37℃ for overnight and LPS (1 μg/ml) at 37℃ for 6 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 60s.

AB-90222-7

Western Blot analysis of various cells using TNF-α Polyclonal Antibody.
Secondary antibody was diluted at 1:20000

AB-90222-2

Immunohistochemistry of paraffinembedded human colon carcinoma using TNF-α Rabbit pAb at dilution of
1:1000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

AB-90222-3

Immunohistochemistry of paraffinembedded human liver using TNF-α Rabbit pAb at dilution of 1:1000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

AB-90222-4

Immunohistochemistry of paraffinembedded human spleen using TNF-α Rabbit pAb at dilution of 1:1000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

AB-90222

Immunofluorescence analysis of A549.
1,primaryAntibody(red) was diluted at 1:200(4°C overnight).
2, GoatAnti Rabbit IgG (H&L) – Alexa Fluor 594 Secondaryantibody was diluted at 1:1000(room temperature,
50min).
3, Picture B: DAPI(blue) 10min.