|Immunogen:||Recombinant protein of human TNF|
|Reactivity:||Hu, Ms, Rt|
Western Blot 1:1000 – 1:2000
|Synonyms:||TNF ; TNFSF2; DIF; Tumor necrosis factor; TNF a; TNF alpha|
TNF-α, the prototypical member of the TNF protein superfamily, is a homotrimeric type-II membrane protein (1,2). Membrane-bound TNF-α is cleaved by the metalloprotease TACE/ADAM17 to generate a soluble homotrimer (2). Both membrane and soluble forms of TNF-α are biologically active. TNF-α is produced by a variety of immune cells including T cells, B cells, NK cells, and macrophages (1). Cellular response to TNF-α is mediated through interaction with receptors TNF-R1 and TNF-R2 and results in activation of pathways that favor both cell survival and apoptosis depending on the cell type and biological context. Activation of kinase pathways (including JNK, Erk (p44/42), p38 MAPK, and NF-κB) promotes the survival of cells, while TNF-α-mediated activation of caspase-8 leads to programmed cell death (1,2). TNF-α plays a key regulatory role in inflammation and host defense against bacterial infection, notably Mycobacterium tuberculosis (3). The role of TNF-α in autoimmunity is underscored by blocking TNF-α action to treat rheumatoid arthritis and Crohn’s disease (1,2,4).
|Buffer:||PBS with 0.02% sodium azide,50% glycerol, pH7.3.|
|Storage:||Store at -20℃. Avoid freeze / thaw cycles.|
Western blot analysis of extracts of various cell lines, using TNF antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human gastric using TNF antibody at dilution of 1:100
Immunofluorescence analysis of RAW264.7 cells using TNF-α antibody at dilution of 1:100. Blue: DAPI for nuclear staining