TOM20
| Cat Number: | MAB-94609 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | EPR15581-54 |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-145 of human TOM20 (NP_055580.1). |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular Weight: | 16kDa |
| Purification: | Aff. Pur. |
| Synonyms: | TOMM20;MAS20;MOM19;TOM20 |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of various cell lines, using TOM20 antibody at 1:1000
dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 60s.
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug TOM20 antibody.
Western blot was performed from the immunoprecipitate using TOM20 antibody at a dilution of 1:1000.
Immunohistochemistry of paraffin embedded human colon using TOM20 antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using TOM20 antibody at dilution of 1:100.
Blue: DAPI for nuclear staining.
Review for TOM20 Rabbit mAb
Experiment Type Immunofluorescence (IF)
Sample: Mouse brown fat cells
References
References for TOM20 Rabbit mAb
Product: TOM20 Rabbit mAb
Journal:Autophagy
Application:WB
IF:11.059
Species:Homo sapiens
PMID:29969932
Title:PTENα regulates mitophagy and maintains mitochondrial quality control.
References for TOM20 Rabbit mAb
Product: TOM20 Rabbit mAb
Journal: Cell Metabolism
Application: IHC
IF: 22.41
Species: Mus musculus
PMID: 31761563
Title: Fructose-1,6-Bisphosphatase 2 Inhibits Sarcoma Progression by Restraining Mitochondrial Biogenesis