Tubulin alpha
| Cat Number: | AB-11661 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-180of human α-Tubulin. |
| Reactivity: | Hu, Ms, Rt, |
| Applications: | Western Blot: 1:2000 – 1:10000 |
| Molecular Weight: | 50kDa |
| Purification: | Aff. Pur. |
| Synonyms: | TUBA4A;ALS22;H2-ALPHA;TUBA1 |
| Background: | Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles |
Western blot analysis of extracts of various cell lines, using α-Tubulin antibody at 1:1000
dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 1s.
Immunofluorescence analysis of C6 cells
using α-Tubulin Rabbit pAb at
dilution of 1:100 (40x lens).
Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3
cells using α-Tubulin Rabbit pAb
at dilution of 1:100 (40x lens).
Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS
cells using α-Tubulin Rabbit pAb
at dilution of 1:100 (40x lens).
Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of
U2OS cells using α-Tubulin antibody
at dilution of 1:100 (60x lens).
Blue: DAPI for nuclear staining.
References
References for α-Tubulin Rabbit pAb
Product:α-Tubulin Rabbit pAb
Journal:Journal of infectious diseases
Application:WB
IF:6.268
Species:Mus musculus
PMID:24652493
Title:Etanercept reduces neuroinflammation and lethality in mouse model of Japanese encephalitis
References for α-Tubulin Rabbit pAb
Product:α-Tubulin Rabbit pAb
Journal:tumor biology
Application:WB
IF:3.649
Species:Homo sapiens
PMID:28347237
Title:Specific tumor-derived CCL2 mediated by pyruvate kinase M2 in colorectal cancer cells contributes to macrophage recruitment in tumor microenvironment