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Tubulin alpha Mouse Monoclonal Antibody

Cat Number: MAB-94273
Conjugate: Unconjugated
Size: 100 ug
Clone: DM1A
Concentration: 1mg/ml
Host: Mouse
Isotype: IgG1
Immunogen: Recombinant protein of human α-Tubulin
Reactivity: Hu, Ms, Rt,
Applications: Western Blot: 1 :500 – 1:5,000 Immunohistochemistry (paraffin embedded tissues): 1:50 – 1:200 Immunofluorescence: 1:20 – 1:100 Immunoprecipitation: 1:20 – 1:50
Molecular Weight: 50kDa
Purification: Aff. Pur.
Synonyms: TUBA4A;ALS22;H2-ALPHA;TUBA1
Background:

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.

Form: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20°C. Avoid freeze / thaw cycles