Tubulin beta Mouse Monoclonal Antibody
| Cat Number: | MAB-80143 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | TUB2 |
| Concentration: | 1mg/ml |
| Host: | Ms |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein of human β-Tubulin |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:5000 – 1:10000 |
| Molecular Weight: | 50kDa |
| Purification: | Aff. Pur. |
| Synonyms: | M40; TUBB1; TUBB5; CDCBM6; CSCSC1; OK/SW-cl.56 |
| Background: | This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,1% BSA,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of various cell lines, using β-Tubulin antibody HRP conj. at 1:5,000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10,000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus Kit.
Exposure time: 1s.
Immunohistochemistry of paraffinembedded rat ovary using β-Tubulin Mouse mAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining
protocol.
Immunohistochemistry of paraffinembedded rat stomach using β-Tubulin Mouse mAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of C6 cells using β-Tubulin Mouse mAb at dilution of 1:100 (40x lens).
Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using β-Tubulin Mouse mAb at dilution of 1:100 (40x lens).
Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using β-Tubulin Mouse mAb at dilution of 1:100 (40x lens).
Blue: DAPI for nuclear staining.
References:
References for β-Tubulin Mouse mAb
Product:β-Tubulin Mouse mAb
Journal:Nucleic acids research
Application:WB, IP
IF:16.971
Species:Mus musculus
PMID:31037292
Title:Zfp217 mediates m6A mRNA methylation to orchestrate transcriptional and post-transcriptional regulation to promote adipogenic differentiation.
References for β-Tubulin Mouse mAb
Product:β-Tubulin Mouse mAb
Journal:Acta Pharmacologica Sinica
Application:WB
IF:6.15
Species:Mus musculus
PMID:31316178
Title:LW-213, a newly synthesized flavonoid, induces G2/M phase arrest and apoptosis in chronic myeloid leukemia.
References for β-Tubulin Mouse mAb
Product:β-Tubulin Mouse mAb
Journal:Virologica Sinica
Application:WB
IF:4.327
Species:Homo sapiens
PMID:31637631
Title:Proteasome Inhibitor PS-341 Effectively Blocks Infection by the Severe Fever with Thrombocytopenia Syndrome Virus
References for β-Tubulin Mouse mAb
Product:β-Tubulin Mouse mAb
Journal:FASEB journal : official publication of the Federation of American Societies for Experimental Biology
Application:WB
IF:5.191
Species:Mus musculus
PMID:31690123
Title:The classic metal-sensing transcription factor MTF1 promotes myogenesis in response to copper
References for β-Tubulin Mouse mAb
Product:β-Tubulin Mouse mAb
Journal:Journal of Cellular and Molecular Medicine
Application:WB
IF:5.31
Species:Rattus norvegicus
PMID:31762213
Title:Proteomics-based screening of the target proteins associated with antidepressant-like effect and mechanism of Saikosaponin A