Tubulin Beta Mouse Monoclonal Antibody
| Cat Number: | MABS15140 |
|---|---|
| Conjugate: | unconjugated |
| Size: | 100 μL |
| Clone: | TUB2 |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG1, κ |
| Immunogen: | Recombinant protein corresponding to Human beta Tubulin |
| Reactivity: | Species, Human, Mouse, Rat |
| Applications: | Applications Dilution WB 1: 1000-1: 2000 ICC/IF 1: 800-1: 1600 |
| Purification: | Affinity purification |
| Synonyms: | Tubb5, Beta 5 tubulin, Class I beta tubulin, Tubulin beta class I, TUBB1, TUBB, TUBB5, Tubulin Beta-5 Chain,β tubulin, CDCBM6 |
| Background: | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. |
| Form: | Liquid |
| Buffer: | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
| Storage: | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
WB analysis of beta Tubulin.Sample: Protein treated by RIPA Lysis Buffer .Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h.Primary antibody: 1: 1000, 4℃ overnight.Secondary antibody: HRP conjugated Goat Anti-Mouse lgG , 1: 5000, RT, 1h.
ICC analysis of beta Tubulinred.Sample: HeLa, 4% PFA 20min.Blocking buffer: 3% BSA in PBS, RT, 30min.Primary antibody: 1: 1000, 4℃ overnight.Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG , 1: 300, RT, 1h.
ICC analysis of beta Tubulinred.Sample: NIH3T3, 4% PFA 20min.Blocking buffer: 3% BSA in PBS, RT, 30min.Primary antibody: 1: 1000, 4℃ overnight.Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG , 1: 300, RT, 1h.