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Tubulin beta

Cat Number: MAB-80143
Conjugate: Unconjugated
Size: 100 ug
Clone: TUB2
Concentration: 1mg/ml
Host: Ms
Isotype: IgG1
Immunogen: Recombinant protein of human Tubulin Beta
Reactivity: Hu, Ms, Rt
Applications:

Western Blot: 1:2000 – 1:10,000

Molecular: 55kDa
Purification: Aff. Pur.
Background:

This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13.

Form: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
MAB-80143-1.jpg

Western blot analysis of extracts of various cell lines, using β-Tubulin antibody HRP conj. at 1:5,000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10,000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus Kit.
Exposure time: 1s.