Tubulin betaIII
| Cat Number: | MAB-10288 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | TU-20 |
| Concentration: | 1mg/ml |
| Host: | Ms |
| Isotype: | IgG1 |
| Immunogen: | Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide. |
| Reactivity: | Hu, Ms, Ch, Bv, Pig, Rt, Ha, Fh |
| Applications: | Flow Cytometry Western Blotting Recommended dilution:1-2 µg/ml, 90 min Positive control: Porcine brain lysate Negative control: HPB-ALL human peripheral blood leukemia cell line Sample preparation: Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Application note: Reducing conditions. Immunohistochemistry (paraffin sections) Recommended dilution: 10 µg/ml Staining technique: Standard ABC technique (DAB+) Pretreatment: 0.1% pepsin (trypsin) in 0.1 M HCl; incubation 30 min in RT; or High temperature citrate buffer antigen retrieval Positive tissue: neuronal tissue Immunocytochemistry Positive material: Neuro2a mouse neuroblastoma cell line |
| Purification: | Aff. Pur. |
| Background: | The tubulin beta III isoform is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.The antibody TU-20 recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human beta III-tubulin. |
| Form: | Liquid |
| Buffer: | Phosphate buffered saline (PBS) solution with 15 mM sodium azide |
| Storage: | Store at 2-8°C. Do not freeze. |