Tubulin betaIII
| Cat Number: | MAB-10288 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | TU-20 |
| Concentration: | 1mg/ml |
| Host: | Ms |
| Isotype: | IgG1 |
| Immunogen: | Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide. |
| Reactivity: | Hu, Ms, Ch, Bv, Pig, Rt, Ha, Fh |
| Applications: | Flow Cytometry |
| Purification: | Aff. Pur. |
| Background: | The tubulin beta III isoform is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.The antibody TU-20 recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human beta III-tubulin. |
| Form: | Liquid |
| Buffer: | Phosphate buffered saline (PBS) solution with 15 mM sodium azide |
| Storage: | Store at 2-8°C. Do not freeze. |
Western Blot analysis using Tubulin Beta III Monoclonal Antibody.
Immunohistochemistry staining of human brain (paraffin sections) using anti-betaIII tubulin (TU-20).
Immunocytochemistry staining of Neuro2a mouse neuroblastoma cell line using anti-betaIII-tubulin (TU-20; green; 3 μg/ml). Nuclei were stained with DAPI (blue).
Immunocytochemistry staining of P-19 mouse embryonal carcinoma cell line stimulated to neuronal differentiation by retinoic acid. A – Microtubules decorated with neuron-specific anti-betaIII-tubulin (TU-20; red). B – Merged image of co-staining with anti-beta-tubulin (TU-06; green; cat. no. 11-251-C100). Superposition of red and green colours provided yellow staining. Nuclei were stained with DNA-binding dye (blue).