Tubulin betaIII

Cat Number: MAB-10288
Conjugate: Unconjugated
Size: 100 ug
Clone: TU-20
Concentration: 1mg/ml
Host: Ms
Isotype: IgG1
Immunogen: Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide.
Reactivity: Hu, Ms, Ch, Bv, Pig, Rt, Ha, Fh

­ Flow Cytometry
­ Western Blotting
Recommended dilution:1-2 µg/ml, 90 min
Positive control: Porcine brain lysate
Negative control: HPB-ALL human peripheral blood leukemia cell line
Sample preparation: Mix lysate with reducing Laemmli SDS-PAGE sample buffer.
Application note: Reducing conditions.
­ Immunohistochemistry (paraffin sections)
Recommended dilution: 10 µg/ml
Staining technique: Standard ABC technique (DAB+)
Pretreatment: 0.1% pepsin (trypsin) in 0.1 M HCl; incubation 30 min in RT; or High
temperature citrate buffer antigen retrieval Positive tissue: neuronal tissue
­ Immunocytochemistry
Positive material: Neuro2a mouse neuroblastoma cell line

Purification: Aff. Pur.

The tubulin beta III isoform is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.The antibody TU-20 recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human beta III-tubulin.

Form: Liquid
Buffer: Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage: Store at 2-8°C. Do not freeze.

Western Blot analysis using Tubulin Beta III Monoclonal Antibody.


Immunohistochemistry staining of human brain (paraffin sections) using anti-betaIII tubulin (TU-20).


Immunocytochemistry staining of Neuro2a mouse neuroblastoma cell line using anti-betaIII-tubulin (TU-20; green; 3 μg/ml). Nuclei were stained with DAPI (blue).


Immunocytochemistry staining of P-19 mouse embryonal carcinoma cell line stimulated to neuronal differentiation by retinoic acid. A – Microtubules decorated with neuron-specific anti-betaIII-tubulin (TU-20; red). B – Merged image of co-staining with anti-beta-tubulin (TU-06; green; cat. no. 11-251-C100). Superposition of red and green colours provided yellow staining. Nuclei were stained with DNA-binding dye (blue).