|Immunogen:||Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide.|
|Reactivity:||Hu, Ms, Ch, Bv, Pig, Rt, Ha, Fh|
The tubulin beta III isoform is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.The antibody TU-20 recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human beta III-tubulin.
|Buffer:||Phosphate buffered saline (PBS) solution with 15 mM sodium azide|
|Storage:||Store at 2-8°C. Do not freeze.|
Western Blot analysis using Tubulin Beta III Monoclonal Antibody.
Immunohistochemistry staining of human brain (paraffin sections) using anti-betaIII tubulin (TU-20).
Immunocytochemistry staining of Neuro2a mouse neuroblastoma cell line using anti-betaIII-tubulin (TU-20; green; 3 μg/ml). Nuclei were stained with DAPI (blue).
Immunocytochemistry staining of P-19 mouse embryonal carcinoma cell line stimulated to neuronal differentiation by retinoic acid. A – Microtubules decorated with neuron-specific anti-betaIII-tubulin (TU-20; red). B – Merged image of co-staining with anti-beta-tubulin (TU-06; green; cat. no. 11-251-C100). Superposition of red and green colours provided yellow staining. Nuclei were stained with DNA-binding dye (blue).