UQCRFS1 Rabbit Polyclonal Antibody

Cat Number: AB-84443
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 141-274 of human UQCRFS1.
Reactivity: Human, Mouse, Rat

Western Blot: 1:500 – 1:2000
Immunocytochemistry 1:50 – 1:200

Molecular: 23KDa
Purification: Affinity purification

[Cytochrome b-c1 complex subunit Rieske, mitochondrial]: Component of the ubiquinolcytochrome
c oxidoreductase, a multisubunit transmembrane complex that is part of the
mitochondrial electron transport chain which drives oxidative phosphorylation. The
respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex
II, CII, ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III,
CIII and cytochrome c oxidase (complex IV, CIV, that cooperate to transfer electrons
derived from NADH and succinate to molecular oxygen, creating an electrochemical
gradient over the inner membrane that drives transmembrane transport and the ATP
synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to
cytochrome c, linking this redox reaction to translocation of protons across the
mitochondrial inner membrane, with protons being carried across the membrane as
hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the
matrix, 4 protons are released into the intermembrane space and 2 electrons are passed
to cytochrome c. The Rieske protein is a catalytic core subunit containing a [2Fe-2S] ironsulfur
cluster. It cycles between 2 conformational states during catalysis to transfer
electrons from the quinol bound in the Q(0 site in cytochrome b to cytochrome c1 (By similarity. Incorporation of UQCRFS1 is the penultimate step in complex III assembly.

Form: Liquid
Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.

Western blot analysis of extracts of various cell lines, using UQCRFS1 Rabbit pAb antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECLWest Pico Plus.
Exposure time: 3s.


Immunohistochemistry of paraffinembedded
rat kidney using UQCRFS1
Rabbit pAb at dilution of 1:100
(40x lens).Perform microwave antigen
retrieval with 10 mM Tris/EDTA buffer pH
9.0 before commencing with IHC staining