|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 461-560 of human SLC17A7.|
Western Blot: 1:200 – 1:2000
The protein encoded by this gene is a vesicle-bound, sodium-dependent phosphate transporter that is specifically expressed in the neuron-rich regions of the brain. It is preferentially associated with the membranes of synaptic vesicles and functions in glutamate transport. The protein shares 82% identity with the differentiation associated Na-dependent inorganic phosphate cotransporter and they appear to form a distinct class within the Na+/Pi cotransporter family.
|Buffer:||PBS with 0.02% sodium azide,50% glycerol,pH7.3.|
|Storage:||For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.|
Western blot analysis of extracts of
SH-SY5Y cells, using VGLUT1
Antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
Detection: ECL West Pico Plus.
Exposure time: 30s.