V5 Tag

Cat Number: MAB-91891
Conjugate: Unconjugated
Size: 100 ug
Clone: S6
Concentration: 1mg/mL
Host: Ms
Isotype: IgG1
Immunogen: A synthetic peptide of Human V5-Tag.
Reactivity: All Species

Western Blot: 1:500-1:5,000
Immunoprecipitation: 1:50-1:200
Immunofluorescence: 1:50-1:200

Purification: Purified

The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

Form: Liquid
Buffer: In PBS (pH7.4), 0.02% Sodium Azide, and 50% glycerol pH7,3.
Storage: Store at 2-8°C for 1 month. Or aliquot and store at -20°C or below for long term. Avoid repeated freeze and thaw cycles.

Western Blot Analysis of recombinant protein
V5 protein with Ms Anti-V5 Tag
At a dilution of 1:2,000
Secondary antibody HRP Goat Anti-Mouse
IgG (H+L) at 1:10,0000
Blocking buffer: 3% notfat dry milk in TBST
Det. ECL West Pico Plus – Exposure: 1s