V5 Tag
| Cat Number: | MAB-91891 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | S6 |
| Concentration: | 1mg/mL |
| Host: | Ms |
| Isotype: | IgG1 |
| Immunogen: | A synthetic peptide of Human V5-Tag. |
| Reactivity: | All Species |
| Applications: | Western Blot: 1:500-1:5,000 |
| Purification: | Purified |
| Background: | The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD). |
| Form: | Liquid |
| Buffer: | In PBS (pH7.4), 0.02% Sodium Azide, and 50% glycerol pH7,3. |
| Storage: | Store at 2-8°C for 1 month. Or aliquot and store at -20°C or below for long term. Avoid repeated freeze and thaw cycles. |
Western Blot Analysis of recombinant protein
V5 protein with Ms Anti-V5 Tag
At a dilution of 1:2,000
Secondary antibody HRP Goat Anti-Mouse
IgG (H+L) at 1:10,0000
Blocking buffer: 3% notfat dry milk in TBST
Det. ECL West Pico Plus – Exposure: 1s