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VAChT (Vesicular Acetylcholine Transporter)

Cat Number: AB-11247
Conjugate: Unconjugated
Size: 100 ul
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human SLC18A3.
Reactivity: Hu, Rt,
Applications:

Western Blot: 1:500 – 1:2000
Immunohistochemistry: 1:50 – 1:200
Immunofluorescence: 1:50 – 1:200

Molecular: 65kDa
Purification: serum
Synonyms: SLC18A3;CMS21;VACHT
Background:

This gene is a member of the vesicular amine transporter family. The encoded transmembrane protein transports acetylcholine into secretory vesicles for release into the extracellular space. Acetylcholine transport utilizes a proton gradient established by a vacuolar ATPase. This gene is located within the first intron of the choline acetyltransferase gene.

Form: Liquid
Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage: For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.
AB-11247-1.jpg

Western blot analysis of extracts of various cell lines, using VACHT Polyclonal Antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 30s.

AB-11247-2.jpg

Immunohistochemistry of paraffin embedded rat brain using VACHT antibody at dilution of 1:200 (40x lens).

AB-11247-3.jpg

Immunofluorescence analysis of mouse brain using VACHT Rabbit pAb at dilution of 1:100 (40x lens).
Blue: DAPI for nuclear staining.