VAChT (Vesicular Acetylcholine Transporter)
| Cat Number: | AB-11247 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ul |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human SLC18A3. |
| Reactivity: | Hu, Rt, |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular Weight: | 65kDa |
| Purification: | serum |
| Synonyms: | SLC18A3;CMS21;VACHT |
| Background: | This gene is a member of the vesicular amine transporter family. The encoded transmembrane protein transports acetylcholine into secretory vesicles for release into the extracellular space. Acetylcholine transport utilizes a proton gradient established by a vacuolar ATPase. This gene is located within the first intron of the choline acetyltransferase gene. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage: | For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of various cell lines, using VACHT Polyclonal Antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 30s.
Immunohistochemistry of paraffin embedded rat brain using VACHT antibody at dilution of 1:200 (40x lens).
Immunofluorescence analysis of mouse brain using VACHT Rabbit pAb at dilution of 1:100 (40x lens).
Blue: DAPI for nuclear staining.