VEGF-A Rabbit Polyclonal Antibody
| Cat Number: | AB-82183 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 200 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human VEGF-A. AA range:110-159 |
| Reactivity: | Human, Mouse, Rat, Pig, Rabbit |
| Applications: | Western Blot: 1/500 – 1/2000 |
| Molecular Weight: | 21kD(monomer),42kD(dimer) |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Synonyms: | VEGFA; VEGF; Vascular endothelial growth factor A; VEGF-A; Vascular permeability factor; VPF |
| Background: | This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. |
| Form: | Liquid |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. and 0.02% sodium azide. |
| Storage: | Store at -20°C. Avoid repeated freeze-thaw cycles. |
Western blot analysis of various lysates, using VEGFA Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 30s.
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Immunofluorescence analysis of A549. 1,primary
Antibody(red) was diluted at 1:200(4°C overnight).
2, Goat Anti Rabbit IgG (H&L) – Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature,
50min)
3, Picture
B: DAPI(blue) 10min.
Immunofluorescence analysis of NIH/3T3 using VEGF antibody at dilution of 1:250. Blue: DAPI for nuclear staining.