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Vimentin Mouse Monoclonal Antibody

Cat Number: MAB-94091
Conjugate: Unconjugated
Size: 100 ug
Clone: 2A52
Concentration: 1mg/ml
Host: Mouse
Isotype: IgG1
Immunogen: Recombinant Protein
Reactivity: Human Rat Mouse
Applications:

WesternBlot: 1/1000-3000
Immunofluorescence: 1/100-200
Immunohistochemistry: 1/200-500

Molecular Weight: 50-57kDa
Purification: Purified
Background:

The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments) intermediate filaments and microtubules.Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells.

Form: Liquid
Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Storage: FStore at -20°C. Avoid repeated freeze-thaw cycles.
MAB-94091-1

Western blot analysis of) Hela 2)Mouse Brain Tissue 3) Rat Brain tissue with EM1159 diluted at:2000.

MAB-94091-2

Western blot analysis of extracts from MCF-7 using Vimentin diluted at 1:3000

MAB-94091-3

Immunohistochemical analysis of paraffin-embedded human breast caricnoma using Vimentin Mouse mAb diluted at:500.

MAB-94091-4

IF analysis of Hela with(Left) and DAPI (Right) diluted at:100.