Vimentin Rabbit Polyclonal Antibody
| Cat Number: | AB-84199 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Vimentin. AA range:56-105 |
| Reactivity: | Hu, Ms |
| Applications: | Western Blot: 1/500 – 1/2000. |
| Molecular Weight: | 50-57kD |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Synonyms: | CTRCT30;HEL113;Vimentin;VIM;vimentin |
| Background: | This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract |
| Form: | Liquid |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage: | Store at -20℃. Avoid repeated freeze-thaw cycles. |
Western blot analysis of extracts of
various cell lines, using Vimentin
antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L)at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST.
Detection: ECL West Pico Plus.
Exposure time: 90s.
Western blot analysis of extracts of
various cell lines, using Vimentin
antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECLWest Pico Plus.
Exposure time: 30s.
Immunohistochemistry of paraffin embedded human lung cancer using Vimentin antibody at dilution
of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using Vimentin antibody at dilution of 1:100. Blue: DAPI for nuclear
staining.