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Vimentin

Cat Number: AB-84199
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human Vimentin.
Reactivity: Hu, Ms
Applications:

Western Blot: 1:500 – 1:2000
Immunohistochemistry: 1:50 – 1:200
Immunofluorescence: 1:50 – 1:200

Molecular: 58kDa
Purification: Purified
Synonyms: CTRCT30;HEL113;Vimentin;VIM;vimentin
Background:

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract

Form: Liquid
Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage: For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.
AB-84199-1.jpg

Western blot analysis of extracts of
various cell lines, using Vimentin
antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L)at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST.
Detection: ECL West Pico Plus.
Exposure time: 90s.

AB-84199-2.jpg

Western blot analysis of extracts of
various cell lines, using Vimentin
antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECLWest Pico Plus.
Exposure time: 30s.

AB-84199-3.jpg

Immunohistochemistry of paraffin embedded
human lung cancer using
Vimentin antibody at dilution
of 1:100 (40x lens).

AB-84199-4.jpg

Immunofluorescence analysis of U2OS
cells using Vimentin antibody at
dilution of 1:100. Blue: DAPI for nuclear
staining.