Vinculin Mouse Monoclonal Antibody
| Cat Number: | MAB-80145 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | VIN-54 |
| Concentration: | 1mg/ml |
| Host: | Mouse |
| Isotype: | IgG1 |
| Immunogen: | vinculin |
| Reactivity: | Human, Rat, Mouse |
| Applications: | Western Blot: 1:2000-1:20000 |
| Molecular Weight: | 124 kDa |
| Purification: | Protein A+G purification |
| Synonyms: | CMD1W, Metavinculin, MVCL, VCL, Vinculin |
| Background: | Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament(F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin(124 kDa), is muscle-specific. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide and 50% glycerol pH 7.3, |
| Storage: | -20℃ for 12 months (Avoid repeated freeze / thaw cycles.) |
Hela lysates were subjected to SDS PAGE followed by western blot with Vinculin Antibody at dilution of 1:5000
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using (Vinculin Antibody) at dilution of 1:200
Immunofluorescent analysis of Hela cells using (vinculin antibody) at dilution of 1:50 and FITC-conjugated Goat Anti-Mouse IgG(H+L)