Vinculin Mouse Monoclonal Antibody

Cat Number: MAB-80145
Conjugate: Unconjugated
Size: 100 ug
Clone: VIN-54
Concentration: 1mg/ml
Host: Mouse
Isotype: IgG1
Immunogen: vinculin
Reactivity: Human, Rat, Mouse

Western Blot: 1:2000-1:20000
Immunohistochemistry: 1:50-1:500
Immunofluorescence: 1:50-1:500

Molecular Weight: 124 kDa
Purification: Protein A+G purification
Synonyms: CMD1W, Metavinculin, MVCL, VCL, Vinculin

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament(F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin(124 kDa), is muscle-specific.

Form: Liquid
Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3,
Storage: -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)

Hela lysates were subjected to SDS PAGE followed by western blot with Vinculin Antibody at dilution of 1:5000


Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using (Vinculin Antibody) at dilution of 1:200


Immunofluorescent analysis of Hela cells using (vinculin antibody) at dilution of 1:50 and FITC-conjugated Goat Anti-Mouse IgG(H+L)