VPS35
| Cat Number: | AB-84318 |
|---|---|
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human VPS35. |
| Reactivity: | Hu, Ms |
| Applications: | WB 1:500 – 1:2000 |
| Molecular Weight: | 92kDa |
| Purification: | Aff. Pur. |
| Synonyms: | VPS35;MEM3;PARK17 |
| Background: | This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of
various cell lines, using VPS35 antibody at 1:1000 dilution._
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)
at 1:10000 dilution._
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST._
Detection: ECL Enhanced Kit.
Exposure time: 60s.