WNT10B
| Cat Number: | AB-84168 |
|---|---|
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant Protein of human WNT10B |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:250 – 1:1000 |
| Molecular Weight: | 43kDa |
| Purification: | Aff. Pur. |
| Synonyms: | WNT10B; SHFM6; STHAG8; WNT-12; protein Wnt-10b |
| Background: | The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It may be involved in breast cancer, and its protein signaling is likely a molecular switch that governs adipogenesis. This protein is 96% identical to the mouse Wnt10b protein at the amino acid level. This gene is clustered with another family member, WNT1, in the chromosome 12q13 region. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide, pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of
HeLa cells, using WNT10B antibody
at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST.
Detection: ECL West Pico Plus.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded
human colon carcinoma
using WNT10B antibody at
dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded
rat ovary using WNT10B
antibody at dilution of
1:100 (40x lens).
Immunofluorescence analysis of C6
cells using WNT10B antibody
at dilution of 1:100. Blue: DAPI for
nuclear staining.
Immunofluorescence analysis of L929
cells using WNT10B antibody at
dilution of 1:100. Blue: DAPI for nuclear
staining.