YAP (phospho Ser127)
| Cat Number: | MAB-94468 |
|---|---|
| Conjugate: | unconjugated |
| Size: | 100 ug |
| Clone: | D9W21 |
| Concentration: | 1 mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human YAP around the phosphorylation site of Ser127. AA range:93-142 |
| Reactivity: | Human;Mouse;Rat;Monkey |
| Applications: | IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
| Molecular: | 65kD |
| Purification: | Affinity purification |
| Synonyms: | YAP1; YAP65; Yorkie homolog; 65 kDa Yes-associated protein; YAP65 |
| Background: | This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2013], |
| Form: | liquid |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5%BSAand0.02% sodium azide |
| Storage: | -20°C/1 year |
Immunofluorescence analysis of Hela cell. 1,YAP (phospho Ser127) Polyclonal Antibody(red) was diluted at 1:200(4° overnight). β-Tubulin Monoclonal Antibody(5G3)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611
Immunofluorescence analysis of rat-heart tissue. 1,YAP (phospho Ser127) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:
Immunofluorescence analysis of rat-heart tissue. 1,YAP (phospho Ser127) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A: