ZEB2
| Cat Number: | AB-81702 |
|---|---|
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1050-1214 of human ZEB2. |
| Reactivity: | Hu,Ms,Rt |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular Weight: | 126kDa |
| Purification: | Aff. Pur. |
| Synonyms: | ZEB2;HSPC082;SIP-1;SIP1;SMADIP1;ZFHX1B |
| Background: | The protein encoded by this gene is a member of the Zfh1 family of 2-handed zinc finger/homeodomain proteins. It is located in the nucleus and functions as a DNAbinding transcriptional repressor that interacts with activated SMADs. Mutations in this gene are associated with Hirschsprung disease/Mowat-Wilson syndrome. Alternatively, spliced transcript variants have been found for this gene. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of HepG2 cells, using ZEB2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in
TBST.
Detection: ECL Enhanced Kit
Exposure time: 3min.
Immunohistochemistry of paraffin embedded
rat brain using ZEB2
antibody at dilution of 1:100
(40x lens).
Immunohistochemistry of paraffin embedded
mouse brain using ZEB2
antibody at dilution of 1:100
(40x lens).
Immunohistochemistry of paraffin embedded
human breast using ZEB2
antibody at dilution of 1:100
(40x lens).
Immunofluorescence analysis of C6 cells
using ZEB2 antibody at dilution
of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3
cells using ZEB2 antibody at
dilution of 1:100. Blue: DAPI for nuclear
staining.