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ZNF281

Cat Number: AB-84194
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 676-895 of human ZNF281.
Reactivity: Hu, Ms, Rt
Applications:

Western Blot: 1:500 – 1:2000
Immunohistochemistry: 1:50 – 1:200

Molecular Weight: 110kDa
Purification: Aff. Pur.
Synonyms: ZNF281; ZBP-99; ZNP-99; zinc finger protein 281.
Form: Liquid
Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
AB-84194-1.jpg

Western blot analysis of extracts of
mouse spleen, using ZNF281
antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 10s.

AB-84194-2.jpg

Western blot analysis of lysates from Hela, A549, MCF-7 cell line, mouse liver, mouse small intestine tissue lysate (from left to right), using ZNF281 Antibody (Center) ZNF281 was diluted at 1: 1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1: 10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.