β Tubulin Rabbit pAb
| Cat Number: | ABE058 |
|---|---|
| Conjugate: | unconjugated |
| Size: | 100 ug |
| Clone: | Polyclonal |
| Concentration: | 1mg/mL |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | Recombinant Protein |
| Reactivity: | Human, Rat, Mouse |
| Applications: | Western Blot 1:2,000-5,000 Immunohistochemistry: 1:500-1,000 |
| Molecular: | 55kDa |
| Synonyms: | Beta 4 tubulin, TBB5, TUBB2, TUBB2A, tubulin beta 2A, beta tubulin, tubulin beta chain |
| Background: | Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used as loading control for these tissues. |
| Form: | liquid |
| Buffer: | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Storage: | Store at -20°C. Avoid repeated freeze-thaw cycles. |
Western blot analysis of 1) Hela, 2)Mouse Brain, 3) Rat Brain tissue with diluted at 1:5,000.
Immunohistochemical analysis of paraffin-embedded Mouse Heart Tissue using β-tubulin Rabbit pAb diluted at 1:500.