53BP1
| Cat Number: | AB-84202 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1763-1977 of human 53BP1 |
| Reactivity: | Hu, Ms, Rt |
| Applications: | WB: 1:500 – 1:2000 |
| Molecular Weight: | 255kDa |
| Purification: | Aff. Pur. |
| Synonyms: | TP53BP1;53BP1;TDRD30;TP53;p202;P53bp1 |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze -thaw cycles. |
Western blot analysis of extracts of A-431 cells, using 53BP1 antibody
at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST. Detection: ECL West Pico Plus.
Exposure time: 40s.
Immunohistochemistry of paraffin embedded rat kidney using 53BP1
antibody at dilution of 1:100
(40x lens).
Immunohistochemistry of paraffin embedded
mouse heart using 53BP1 antibody at dilution of 1:100
(40x lens).