Cat Number: AB-84202
Conjugate: Unconjugated
Size: 100ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1763-1977 of human 53BP1
Reactivity: Hu, Ms, Rt

WB: 1:500 – 1:2000
IHC: 1:50 – 1:200
IF: 1:50 – 1:200

Molecular Weight: 255kDa
Purification: Aff. Pur.
Synonyms: TP53BP1;53BP1;TDRD30;TP53;p202;P53bp1
Form: Liquid
Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze -thaw cycles.

Western blot analysis of extracts of A-431 cells, using 53BP1 antibody
at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST. Detection: ECL West Pico Plus.
Exposure time: 40s.


Immunohistochemistry of paraffin embedded rat kidney using 53BP1
antibody at dilution of 1:100
(40x lens).


Immunohistochemistry of paraffin embedded
mouse heart using 53BP1 antibody at dilution of 1:100
(40x lens).