PBEF Rabbit Polyclonal Antibody
| Cat Number: | AB-E12102 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | Synthesized peptide derived from human PBEF. at AA range: 411-460 |
| Reactivity: | Human;Mouse;Rat |
| Applications: | Western Blot: 1:500-2000 Immunohistochemistry (paraffin-embedded tissues): 1:50-300 |
| Molecular Weight: | 54kDa |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Synonyms: | Nicotinamide phosphoribosyltransferase (NAmPRTase) (Nampt) (EC 2.4.2.12) (Pre-B-cell colony-enhancing factor 1) (Pre-B cell-enhancing factor) (Visfatin |
| Background: | This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yieldnicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide.The protein belongs to the nicotinic acid |
| Form: | Liquid |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage: | Store at -20°C. Avoid repeated freeze-thaw cycles. |
Western Blot analysis of 1,mouse-lung 2,mouse-kidneycells using primary antibody diluted at 1:500(4°Covernight). Secondary antibody:Goat Anti-rabbit IgG
IRDye 800( diluted at 1:5000, 25°C, 1 hour)
Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was
used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).