ACKR3
| Cat Number: | AB-84309 |
|---|---|
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human ACKR3 |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular Weight: | 55KkDa |
| Purification: | Aff. Pur. |
| Synonyms: | ACKR3;CMKOR1;CXC-R7;CXCR-7;CXCR7;GPR159;RDC-1;RDC1 |
| Background: | This gene encodes a member of the G-protein coupled receptor family. Although this protein was earlier thought to be a receptor for vasoactive intestinal peptide (VIP), it is now considered to be an orphan receptor, in that its endogenous ligand has not been identified. The protein is also a coreceptor for human immunodeficiency viruses (HIV). Translocations involving this gene and HMGA2 on chromosome 12 have been observed in lipomas. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage: | Store at -20°C, and avoid repeat freeze-thaw cycles. |
Western blot analysis of extracts of
various cell lines, using ACKR3
antibody at 1:3000 dilution.
Secondary antibody: HRP
Goat Anti-Rabbit IgG (H+L)
at 1:10000 dilution. Lysates/proteins:
25ug per lane._Blocking buffer: 3%
nonfat dry milk in TBST._Detection:
ECL West Pico Plus.
Exposure time: 90s.
Immunofluorescence analysis of
Mouse skin using ACKR3 Rabbit pAb
at dilution of 1:100. Blue:
DAPI for nuclear staining.