AFP
| Cat Number: | AB-84139 |
|---|---|
| Size: | 50 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant protein of human AFP. |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular Weight: | 68kDa |
| Purification: | Aff. Pur. |
| Synonyms: | AFP Alpha-fetoprotein antibodyAlpha-1-fetoprotein antibody Alphafetoglobulin AFP antibody HPAFP. |
| Background: | Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of mouse liver,
using AFP antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG
(H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s
Immunofluorescence analysis