AKT1 Mouse Monoclonal Antibody
| Cat Number: | MAB-94664 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | 8H0 |
| Concentration: | 1mg/ml |
| Host: | Mouse |
| Isotype: | IgG1 |
| Immunogen: | synthetic peptide derived from AKT |
| Reactivity: | Hu, Ms , Rt |
| Applications: | Western Blot: 1:1000-1:10000 |
| Molecular Weight: | 56-62 kDa |
| Purification: | Protein A+G purification |
| Synonyms: | AKT, AKT1, AKT2, AKT3, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha |
| Background: | The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. This antibody detects all the members of AKT with/without phospho-modification. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide and 50% glycerol pH 7.3 |
| Storage: | Store -20℃ for 12 months (Avoid repeated freeze / thaw cycles.) |
HEK-293 cells were subjected to SDS PAGE followed by western blot with AKT Antibody at dilution of 1:2000
Mouse brain tissue were subjected to SDS PAGE followed by western blot with AKT Antibody at dilution of 1:1000
IP Result of anti-AKT1 IP 3ug; Detection: 1:500) with HeLa cells lysate 2500ug.
Immunohistochemistry of paraffin-embedded human breast cancer using (AKT antibody) at dilution of 1:200