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Alexa Fluor 405, Donkey Anti Rat IgG (H+L)

Cat Number: IS20419
Size: 1 mg
Clone: POLY
Concentration: 2mg/ml
Host: Donkey
Isotype: IgG
Reactivity: Rat
Applications:

Fluorescence microscopy: 1-2 ug/mL
FC: 1 ug/106 cells
Near-infrared western detection: 50-100 ng/mL

Purification: Aff. Pur.
Background:

ALEXA FLUOR 405 antibodies are affinity-purified antibodies labeled with a blue fluorescent dye Alexa Fluor 405, one of an outstanding series of Alexa Fluor dyes developed by IS. Alexa Fluor dyes are superior to other commercially available dyes for antibody labeling by having combined advantages in brightness, photostability, and specificity

Form: liquid
Buffer: pH~7.4 PBS containing 50% glycerol, 2 mg/ml bovine serum albumin (IgG-free and protease-free) and 0.05% sodium azide.
Storage: Store at 4°C for several months. Protect from light. For longer storage, divide the conjugate into small aliquots and freeze at -20°C. Avoid repeated freezing and thawing.
Properties: Spectral Properties λAbs/λEm = 408/452 nm (in pH 7.4 PBS buffer) ALEXA FLUOR 405 is spectrally similar to Pacific Blue®.