Alkaline Phopshatase
| Cat Number: | AB-84118 |
|---|---|
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant protein of human ALPL |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:2500 -1:5000 |
| Molecular Weight: | 70kDa |
| Purification: | Aff. Pur. |
| Synonyms: | ALPL; AP-TNAP; APTNAP; HOPS; TNAP; TNSALP; alk aline phosphatase, liver/bone/kidney |
| Background: | This gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placentallike, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme may play a role in bone mineralization. Mutations in this gene have been linked to hypophosphatasia, a disorder that is characterized by hypercalcemia and skeletal defects. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of various cell lines, using ALPL antibody
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human kidney using ALPL antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of SW480 cells using ALPL antibody.
Immunofluorescence analysis of HeLa cells using ALPL antibody.