AMBRA1
| Cat Number: | AB-83947 |
|---|---|
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1109-1208 of human AMBRA1 |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500 – 1:1000 |
| Molecular Weight: | 143kDa |
| Purification: | Aff. Pur. |
| Synonyms: | AMBRA1; DCAF3; WDR94; autophagy and beclin 1 regulator 1 |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of various cell lines, using AMBRA1 Antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus. Exposure time: 60s.
Immunohistochemistry of paraffin-embedded mouse stomach using AMBRA1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using AMBRA1 antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using AMBRA1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.