Ampk Alpha1 / PRKAA1
| Cat Number: | AB-84049 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human AMPK1. AA range:451-500 |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1/500 – 1/2000 |
| Molecular Weight: | 65kDa |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Synonyms: | PRKAA1; AMPK; AMPKa1; protein kinase AMP-activated catalytic subunit alpha 1 |
| Background: | The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5′-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATPconsuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed |
| Form: | Liquid |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage: | Store at -20°C. Avoid repeated freeze-thaw cycles. |
Western Blot analysis of mouse-lung cells using primary
antibody diluted at 1:1000(4°C overnight). Secondary
antibody:Goat Anti-rabbit IgG IRDye 800( diluted at
1:5000, 25°C, 1 hour). Cell lysate was extracted by
Minute™ Plasma Membrane Protein Isolation
Immunofluorescence analysis of rat-lung tissue.
1,AMPKα1 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight).
2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).
3, Picture B: DAPI(blue) 10min.
Picture A:Target.
Immunofluorescence analysis of rat-lung tissue.
1,AMPKα1 Polyclonal Antibody(red) was diluted at
1:200(4°C,overnight). 2, Cy3 labled Secondary antibody
was diluted at 1:300(room temperature, 50min).3, Picture
B: DAPI(blue) 10min.
Picture A:Target.
Picture B: DAPI.
Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,AMPKα1 Polyclonal Antibody was diluted at 1:200(4°C,overnight).
2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min).
3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
Negative control was used by secondary antibody only.